bmrB [2018-10-26 13:18:33]

regulatory leader peptide for the control of bmrC-bmrD expression

locus: BSU09700

pI: 11.08

mw: 6.18 kDa

protein length: 53 aa

gene length: 159 bp

function: control of bmrC-bmrD expression

product: leader peptide

essential: no

synonyms: yheJ

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.9|Other regulators]

Gene

Coordinates: 1,045,037 → 1,045,198

Expression and Regulation

Operons

• genes: [gene|E9956A958C66C4114EB974EA6F9BF30CD951034A|bmrB]-[gene|67C0C9EA17EF953554D3D57CD0D285246F50C1AD|bmrC]-[gene|9B2766D2F8892AA17DB480216570C81C33355C3B|bmrD]
  description: [Pubmed|25217586]

  regulatory mechanism

  • [protein|E5110D9C36E8C55AFD1419987B14A53232165F20|AbrB]: repression, [Pubmed|20817675,25217586], in [regulon|E5110D9C36E8C55AFD1419987B14A53232165F20|AbrB regulon]
  • [protein|E9956A958C66C4114EB974EA6F9BF30CD951034A|BmrB]: attenuation, [Pubmed|25217586], in [regulon|E9956A958C66C4114EB974EA6F9BF30CD951034A|BmrB regulon]

  regulation

  • ''[protein|search|bmrB]-[protein|search|bmrC]-[protein|search|bmrD]'' expression only occurs during the late-exponential and stationary growth stages ([protein|search|AbrB]) [Pubmed|25217586]

Biological materials

Mutant

• MGNA-A706 (yheJ::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/706 NBRP B. subtilis, Japan]
• BKE09700 (Δ[gene|E9956A958C66C4114EB974EA6F9BF30CD951034A|bmrB]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE09700 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACGCAAATTCCTTGGCATA, downstream forward: _UP4_TAAGGAAAGACAGGTGTATG
• BKK09700 (Δ[gene|E9956A958C66C4114EB974EA6F9BF30CD951034A|bmrB]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK09700 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACGCAAATTCCTTGGCATA, downstream forward: _UP4_TAAGGAAAGACAGGTGTATG

References

• 20817675,25217586